The approaches and challenges in delivery CRISPR/Cas9

Abstract

A significant technological advance, grouped regularly separated short palindromic repeats/CRISPR-associated protein 9 genome editing program has revolutionized genetic modification for precision medicine and therapeutic and diagnostic applications. Furthermore, efficient transport of the CRISPR elements is necessary for the successful application of this type of gene editing for therapeutics. However, there are considerable challenges associated with delivering CRISPR/Cas9 to the target. The CRISPR/Cas9 gene editing system's molecular mechanisms, current delivery strategies, and the various CRISPR/Cas9 delivery vehicles, including non-viral delivery methods like microinjection and electroporation and this review will address virus transmission strategies such as adeno-associated virus (AAV) and CRISPR-Phage, as well as a discussion of their specific advantages. At last, we discuss major obstacles to CRISPR/Cas9 efficacy that must be solved before successful human gene therapy may be achieved.